Heme Oxygenase-1 (HO-1) also known as Hsp32, is the inducible isoform of heme oxygenase that catalyzes the NADPH, oxygen, and cytochrome P450 reductase dependent oxidation of heme to carbon monoxide, ferrous iron and biliverdin which is rapidly reduced to bilirubin. These products of the HO reaction have important physiological effects: carbon monoxide is a potent vasodilator and has been implicated to be a physiological regulator of cGMP and vascular tone; biliverdin and its product bilirubin are potent antioxidants; "free" iron increases oxidative stress and regulates the expression of many mRNAs (e.g., DCT-1, ferritin and transferring receptor) by affecting the conformation of iron regulatory protein (IRP)-1 and its binding to iron regulatory elements (IREs) in the 5'- or 3'- UTRs of the mRNAs. To date, three identified heme oxygenase isoforms are part of the HO system that catalyze heme into biliverdin and carbon monoxide. These are inducible HO-1 or Hsp32, constitutive HO-2 that is abundant in the brain and testis, and HO-3 which is related to HO-2 but is the product of a different gene. The HO system is the rate-limiting step in heme degradation and HO activity decreases the levels of heme which is a well known potent catalyst of lipid peroxidation and oxygen radical formation.

Alternative Name:?HMOX1, Hsp32, Heat shock protein 32, Heme oxygenase 1

Clone:?HO-1-1

Host:?Mouse

Isotype:?IgG1κ

Immunogen:?Synthetic peptide corresponding to the sequence near the N-terminus of human HO-1.

UniProt ID:?P09601

GenBank ID:?X14782

Gene/Protein Identifier:?3162 (Entrez GeneID), 141250 (OMIM)

Source:?Purified from ascites.

Species reactivity:?Human, Mouse, Rat

Bovine, Dog

Applications:?Flow Cytometry, ICC, IHC (PS), WB

Recommended Dilutions/Conditions:?Flow Cytometry (10μg/ml)

Western Blot (1:1,000 ECL)

Suggested dilutions/conditions may not be available for all applications.

Optimal conditions must be determined individually for each application.

Application Notes:?Detects a band of ~32kDa by Western blot.

Purity Detail:?Protein G affinity purified.

Formulation:?Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.

Shipping:?Shipped on Blue Ice

Long Term Storage:?-20°C

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血紅素加氧酶-1 （HO-1） 也稱為 Hsp32，是血紅素加氧酶的誘導(dǎo)亞型，可催化血紅素的 NADPH、氧氣和細(xì)胞色素 P450 還原酶依賴性氧化為一氧化碳、亞鐵和膽綠素，并迅速還原為膽紅素。HO反應(yīng)的這些產(chǎn)物具有重要的生理作用：一氧化碳是一種有效的血管擴(kuò)張劑，并且被認(rèn)為是cGMP和血管張力的生理調(diào)節(jié)劑;膽綠素及其產(chǎn)物膽紅素是有效的抗氧化劑;“游離”鐵通過影響鐵調(diào)節(jié)蛋白（IRP）-1的構(gòu)象及其與mRNA的1'或5'-UTR中的鐵調(diào)節(jié)元件（IRE）的結(jié)合，增加氧化應(yīng)激并調(diào)節(jié)許多mRNA（例如DCT-3，鐵蛋白和轉(zhuǎn)移受體）的表達(dá)。

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艾美捷血紅素加氧酶-1(HO-1) 單克隆抗體（ADI-OSA-110-D）相關(guān)參數(shù)：

替代名稱：?HMO

免疫原：?對應(yīng)于人HO-1N末端附近序列的合成肽。

基因/蛋白質(zhì)標(biāo)識符：?3162 （Entrez GeneID）， 141250 （OMIM）

源：?從腹水中純化。

物種反應(yīng)性：?人類，?老鼠，?鼠牛，?狗

應(yīng)用：?流式細(xì)胞術(shù)，?ICC， IHC （PS）， WB

推薦稀釋度/條件：?流式細(xì)胞術(shù)（10μg/ml）蛋白質(zhì)印跡（1：1，000 ECL）

建議的稀釋度/條件可能不適用于所有應(yīng)用。

應(yīng)用說明：?通過蛋白質(zhì)印跡檢測~32kDa的條帶。

純度細(xì)節(jié)：?蛋白G親和純化。

配方：?液。在含有50%甘油和0.09%疊丨氮丨化鈉的PBS中。

航運(yùn)：?在藍(lán)冰上運(yùn)輸

長期儲存：?-20°C

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圖：HO-1?（Hsp32）、單克隆抗體 （HO-1-1） 的蛋白質(zhì)印跡分析（產(chǎn)品編號ADI-OSA-110）：車道?1：MW?標(biāo)記;泳道?2：HO-1（大鼠）、（重組）（產(chǎn)品編號ADI-SPP-730）;車道?3：HO-1（人）、（重組）（產(chǎn)品編號ADI-SPP-732）;車道?4：HO-2（人）、（重組）（產(chǎn)品編號ADI-SPP-550）;泳道5：MDBK細(xì)胞裂解物;車道6：狗肝微粒體;車道7：小鼠肝臟微粒體。

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血紅素加氧酶-1(HO-1) 單克隆抗體（ADI-OSA-110-D）部分文獻(xiàn)參考：

A preliminary attempt to establish multiple stress response profiles of human skin fibroblasts exposed to mild or severe stress during ageing in vitro: S.I.S. Ratten, et al.; Mech. Ageing Dev. 170, 92 (2018);

Cinnamic aldehyde inhibits vascular smooth muscle cell proliferation and neointimal hyperplasia in Zucker Diabetic Fatty rats: N.E. Buglak, et al.; Redox Biol. 19, 166 (2018);

Dysregulated heme oxygenase-1low M2-like macrophages augment lupus nephritis via Bach1 induced by type I interferons: D. Kishimoto, et al.; Arthritis Res. Ther. 20, 64 (2018);

Dysregulation of cardiac lipid parameters in high-fat high-cholesterol diet-induced rat model: Q. Han, et al.; Lipids Health Dis. 17, 255 (2018);

Loss of heme oxygenase-1 accelerates mesodermal gene expressions during embryoid body development from mouse embryonic stem cells: Y.L. Lai, et al.; Redox Biol. 15, 51 (2018);

Lutzomyia longipalpis Saliva Induces Heme Oxygenase-1 Expression at Bite Sites: N.F. Luz, et al.; Front. Immunol. 9, 2779 (2018);

Neuroprotective effect of an Nrf2-ARE activator identified from a chemical library on dopaminergic neurons: Y. Izumi, et al.; Eur. J. Pharmacol. 818, 470 (2018),