Acridine Orange 10-Nonyl Bromide,線粒體熒光探針（10-壬基溴代吖啶橙）是一種異染性染料，可用作活細(xì)胞中的線粒體熒光探針。 它已被用于通過流式細(xì)胞術(shù)分析線粒體、表征多藥耐藥性以及測量大鼠胸腺細(xì)胞凋亡過程中線粒體質(zhì)量的變化。熒光 :λex 496 nm, λem 524 nm in CH3OH.

10-壬基溴代吖啶橙 Acridine Orange 10-Nonyl Bromide (75168-11-5) 應(yīng)用：
Acridine Orange 10-nonyl bromide is a metachromatic dye useful as a mitochondrial probe in living cells. It has been used to analyze mitochondria by flow cytometry, characterize multidrug resistance, and measure changes in mitochondrial mass during apoptosis in rat thymocytes.
10-壬基吖啶橙(NAO)是吖啶橙的衍生物, 是一種用于完整細(xì)胞內(nèi)線粒體特異性熒光標(biāo)記物。與Rh123（Mb1811）相比，細(xì)胞中NAO的積聚與質(zhì)子動力驅(qū)動關(guān)系不大，但是與線粒體相關(guān)膜蛋白或者脂類發(fā)生作用相關(guān)。NAO的攝入不依賴于線粒體跨膜電勢差，可用于檢測線粒體。NAO可與心磷脂等帶負(fù)電荷磷脂特異性結(jié)合, 其與線粒體膜的相互作用不依賴于線粒體的膜電位; 分析細(xì)胞凋亡時(shí), 可用羅丹明123來檢測線粒體的膜電位, 而用NAO來檢測線粒體結(jié)構(gòu)的完整性。NAO和羅丹明123（Rh123）是研究線粒體功能一對好的染料。

案例：

A: Fluorescence microscopy of Acridine orange 10-nonyl bromide (NAO) stained mitochondria (pseudo coloured red) in spongy myocardium. The segmentation masks are shown as green lines around the mitochondria. B: Quantification of fluorescence intensity inside the segmentation masks showed that mitochondrial area within the cardiomyocytes of Atlantic salmon was significantly increased by the high-intensity training regime (approx. 16000 mitochondrial from sections of 5 and 6 hearts from control and high-intensity regimes, respectively). The number of mitochondria per cell though, was not significantly different as shown by that the ratio between mitochondrial DNA and genomic DNA (mtDNA/gDNA). *: Student’s t-test p<0.05, performed on metric and log2ER values for area and mtDNA/gDNA respectively. n?=?12/group.