艾美捷?RmesFect CRISPR轉(zhuǎn)染試劑#RMC70500專為mRNA/gRNA轉(zhuǎn)染而設(shè)計(jì)，其具有如下特點(diǎn)：

高效：在所有細(xì)胞中實(shí)現(xiàn)RNA遞送

即用型：無(wú)需額外的緩沖液

低核酸量：毒性最小化

保護(hù)?RNA 免受降解

與任何培養(yǎng)基兼容

血清兼容：無(wú)需更換培養(yǎng)基

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RmesFect? CRISPR transfection Reagent based on the TEE-technology is specifically designed for mRNA/gRNA transfection with high efficiency and low toxicity. RmesFect? is efficient in a large variety of cells.

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Benefits:

Highly efficient: achieve RNAs delivery in all cells

Ready-to-use: no need for additional buffer

Low nucleic acid amount : minimized toxicity

Protects RNAs against degradation

Compatible with any culture medium

Serum compatible : Medium changed not required

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與質(zhì)粒DNA遞送相比，mRNA轉(zhuǎn)染具有以下優(yōu)勢(shì)：

無(wú)需核吸收。mRNA翻譯成蛋白質(zhì)發(fā)生在細(xì)胞質(zhì)中。

蛋白表達(dá)速度快于DNA轉(zhuǎn)染

無(wú)基因組整合

以完全啟動(dòng)子非依賴的方式表達(dá)蛋白質(zhì)

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RmesFect CRISPR轉(zhuǎn)染試劑結(jié)果示意圖：

根據(jù)標(biāo)準(zhǔn)方案，使用RmesFect CRISPR用編碼GFP和RFP的mRNA（各0.25μg）轉(zhuǎn)染DC24細(xì)胞。轉(zhuǎn)染后24小時(shí)在顯微鏡熒光下分析圖像。