學(xué)習(xí)順序與主題 LEARNING SEQUENCES AND TOPICS

4．運(yùn)輸機(jī)制

4.1 蛋白質(zhì)的命途

4.2 信號假說

4.3 共翻譯轉(zhuǎn)移

4.4 翻譯后轉(zhuǎn)移

4.5 拓?fù)浣Y(jié)構(gòu)

5. 細(xì)胞隔間運(yùn)輸

5.1 囊泡運(yùn)輸

5.2 脈沖追蹤技術(shù)

5.3 內(nèi)質(zhì)網(wǎng)定位信號KDEL

5.4 囊泡的定向出芽與融合

5.5 外殼蛋白

5.6 Rabs 與 SNAREs

6. 胞吞與胞吐

6.1 胞吞胞吐概述

6.2 胞吞作用的類型

6.3 膽固醇的運(yùn)輸（1）概述

6.4 膽固醇的運(yùn)輸（2）步驟

6.5 如何量化內(nèi)吞速率？

目標(biāo) GOALS

Review how the steps of translation, post-translational modifications, and folding map to distinct locations in the cell.

了解翻譯、翻譯后修飾和折疊如何映射到細(xì)胞的不同位置。

Understand the processes involved in discrete steps of protein maturation and transport.

了解蛋白質(zhì)成熟和運(yùn)輸?shù)莫?dú)立步驟及其所涉及的過程

Understand how specific molecular machinery is essential to protein transport across cellular compartments.

了解特定的分子機(jī)制為何對跨細(xì)胞隔間的蛋白質(zhì)轉(zhuǎn)運(yùn)至關(guān)重要。

Detail the molecular mechanisms of protein trafficking to membranes.

詳細(xì)說明蛋白質(zhì)向膜運(yùn)輸?shù)姆肿訖C(jī)制。

你應(yīng)該能做到 You should be able to

1.????? Identify the cellular locations of different biochemical and spatial steps during protein trafficking.?

2.????? Interpret the results of a pulse-chase experiment.

3.????? Design an experiment to determine the location of the signal for protein transport.

4.????? Explain the significance of the signal sequence.?

5.????? Detail the models (cotranslational vs. post-translational translocation) for protein entry into the secretory pathway.

6.????? Describe the structure and function of the signal recognition particle (SRP), the SRP receptor, and the Sec61 channel (translocon)

7.????? Explain how protein folding and covalent modifications are interdependent.

8.????? Summarize general features of protein glycosylation throughout the secretory pathway.

9.????? Identify the functions of different classes of proteins, such as chaperones and coats, in protein trafficking.?

10.??? Propose a mechanism for how proteins move between cellular compartments depending on their final cellular destination.?

11.??? Describe the GTPase, GAP, and GEF mechanisms important in protein trafficking.

12.??? Propose a role for SNAREs and Rabs in vesicle movement.

13.??? Explain the roles of protein trafficking, exocytosis, and endocytosis in regulating cellular processes.?

14.??? Summarize the mechanism of clathrin-mediated endocytosis, as exemplified by cholesterol uptake.

15.??? Describe how viruses can utilize host cell endocytosis and exocytosis pathways throughout their life cycles.