經(jīng)典案例|普健哺乳動物細(xì)胞表達(dá)系統(tǒng)之scfv表達(dá)
重組蛋白表達(dá)系列一
哺乳動物細(xì)胞表達(dá)系統(tǒng)
展示案例：HEK293F Expression System
?Project Information?
（1）Protein Name: protein L（一種scfv）
該蛋白不易表達(dá)，采用哺乳動物細(xì)胞表達(dá)系統(tǒng)表達(dá)活性最佳，其他表達(dá)系統(tǒng)表達(dá)活性低。
（2）Expression vector: pATX2
（3）Expression host: HEK293F
總結(jié)：蛋白L（scfv）采用普健高表達(dá)pATX系列載體配合公司優(yōu)化高密度哺乳表達(dá)系統(tǒng)Xten293，成功表達(dá)，表達(dá)量高達(dá)31.2mg/L，純度>90%，經(jīng)檢驗(yàn)，其具有較高活性。
以圖為鑒
Test Report
01
Expression test
MW. Molecular weight marker. ?. Non-transfected (negative control).
The protein L expressed secretorily?in HEK293F cells.
02
Purification Test
MW. Molecular weight marker. IN. Input. ?FT. Flow through. W. Washes. E. Eluted fractions.
A. The purification test result. B. Final QC gel.
L-pATX2 was transfected with 80ml HEK293F cells. The culture medium and cells which were collected on the sixth day?post transfection did purification test.
03
Fermentation and Purification
1L scale up purification
MW. Molecular weight marker. IN. Input. ?FT. Flow through. W. Washes. E. Eluted fractions.
A. The purification test result. B. Final QC gel.
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