學習順序與主題 LEARNING SEQUENCES AND TOPICS

07 核孔

7.1 核被膜概述

7.2 核孔復合體的組成與結構

7.3 不同運輸方式比較

08 細胞核轉運的分子機制

8.1 如何確定 NLS 與 NES

8.2 核轉運受體

8.3 Ran-GTPase與跨核膜運輸

09 核運輸?shù)臋z測

9.1 熒光共振能量轉移 (FRET)——光譜重疊

9.2 熒光共振能量轉移 (FRET)——Ran梯度

9.3 活化T細胞核內(nèi)因子-鈣調(diào)神經(jīng)磷酸酶途徑 (NFAT-CaN途徑)

目標 GOALS

1.?Understand how the structure of the nuclear pore complex influences transport in and out of the nucleus.

了解核孔復合體的結構如何影響細胞核跨膜運輸。

2.?Detail the interactions between molecular machinery that facilitates nuclear transport.

詳細說明促進細胞核跨膜運輸?shù)姆肿酉嗷プ饔脵C制。

你應該能做到 You should be able to

Contrast movement of proteins in nuclear transport to those in membrane trafficking.

對比核轉運和膜轉運中的蛋白運動。

Explain how the structures of the nuclear envelope and nuclear pore complex affect nuclear transport.

解釋核被膜與核孔復合體的結構如何影響核運輸。

Determine how molecules will be transported in and out of the nucleus based on their cellular function.

根據(jù)細胞功能，確定分子如何進出細胞核。

Experimentally determine nuclear localization and export sequences.

實驗確定核定位和輸出序列。

Explain the function of nuclear localization and export sequences.

解釋核定位和輸出序列的功能。

Identify the required proteins for nuclear transport.

確定細胞核運輸所需的蛋白質。

Summarize the steps for nuclear import and export.

總結蛋白質進出細胞核的步驟。

Describe how the cell establishes and maintains Ran asymmetry.

描述細胞如何建立和維持Ran不對稱性。

Propose experiments to characterize the proteins involved in nuclear transport.

設計實驗來表征參與核運輸?shù)牡鞍踪|。

Explain how F?rster (or Fluorescence) Resonance Energy Transfer (FRET) can be used to study the location and interactions of proteins.

解釋熒光共振能量轉移技術（FRET）如何用于研究蛋白質的位置和相互作用。

Interpret a FRET experiment.

解釋FRET實驗。

Predict how specific post-translational modifications regulate nuclear transport.

預測特定的翻譯后修飾如何調(diào)節(jié)核運輸。

Explain why maintaining high specificity and rapid flux through the nuclear pore is especially perplexing.

解釋為什么通過核孔保持高特異性和快速通量特別令人困惑。