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定量測(cè)定多物種PGE2——艾美捷PGE2酶聯(lián)免疫試劑盒方案

2023-01-10 16:22 作者:艾美捷  | 我要投稿

Prostaglandin E2 (PGE2) is an extensively studied prostaglandin owing to its predominance in inflammation, cancer, atherosclerosis, autoimmune disease, and sepsis. Oxidation of arachidonic acid by prostaglandin synthases (COX-1 and COX-2) produces prostaglandin H2 (PGH2), which is further metabolized by PGE synthases into its major product, PGE2. PGE2 mediates autocrine and paracrine signaling by binding to G-protein coupled receptors (EP1, EP2, EP3, EP4) on the cell surface, functioning to modulate phospholipase C and adenylate cyclase activity. PGE2 has been of great interest as a therapeutic target, either by modulation of its synthesis by COX inhibitors (NSAIDS) or by modulation of its receptors by downregulation or binding antagonists. PGE2 production in a variety of tissues has been shown to modulate numerous physiological processes including natriuresis in the kidney, smooth muscle elasticity in the vasculature, and the inflammatory response to damaged tissues by monocytes and macrophages.

Sensitivity:?13.4 pg/ml (range 39.1 - 2,500 pg/ml)

Assay Time:?<3 hours

Applications:?ELISA, Colorimetric detection

Application Notes:?For the quantitative determination of PGE2 in culture supernatants, serum, saliva, urine, and whole blood from any species. Cited sample types include cerebral spinal fluid, dialysate, gingival crevicular fluid, peritoneal exudate fluid and peritoneal exudate cell supernatant, plasma, and tissue.

Wavelength:?405 nm

Species reactivity:?Species independent

Use/Stability:?Store all components at +4°C, except standard and conjugate at -20°C.

Shipping:?Shipped on Blue Ice

Contents:?GxM IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution

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PGE2酶聯(lián)免疫試劑盒:用于炎癥和類二十烷研究的高靈敏度PGE2 ELISA試劑盒。

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PGE2酶聯(lián)免疫試劑盒化學(xué)性質(zhì):

替代名稱:前列腺素E2

靈敏度:13.4 pg/ml(范圍39.1-2500 pg/ml)

化驗(yàn)時(shí)間:<3小時(shí)

應(yīng)用:ELISA,比色檢測(cè)

應(yīng)用說(shuō)明:用于定量測(cè)定任何物種的培養(yǎng)上清液、血清、唾液、尿液和全血中的PGE2。引用的樣本類型包括腦脊髓液、透析液、齦溝液、腹膜滲出液和腹膜滲出細(xì)胞上清液、血漿和組織。

波長(zhǎng):405 nm

物種反應(yīng)性:物種無(wú)關(guān)

使用/穩(wěn)定性:在+4°C下儲(chǔ)存所有成分,-20°C下標(biāo)準(zhǔn)和共軛物除外。

運(yùn)輸:藍(lán)冰運(yùn)輸

內(nèi)容:GxM IgG微量滴定板、綴合物、抗體、測(cè)定緩沖液、洗滌緩沖液濃縮液、標(biāo)準(zhǔn)品、pNpp底物、停止溶液

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PGE2酶聯(lián)免疫試劑盒文獻(xiàn)參考:

Leptin reduces in vitro cementoblast mineralization and survival as well as induces PGE2 release by ERK1/2 commitment: G. Ruiz-Heiland, et al.; Clin. Oral. Investig. 25, 1933 (2021), Abstract; Full Text

Potential Surrogate Quantitative Immunomodulatory Potency Assay for Monitoring Human Umbilical Cord-derived Mesenchymal Stem Cells Production: S. Teng, et al.; Cell Biol. Int. 45, 1072 (2021), Abstract;

Synergistic effect of Korean red ginseng extract and GABA mixture on the IgE production in mice via Th1/Th2 cell balance: J.S. Lim, et al.; Food Sci. Biotechnol. 30, 1571 (2021), Abstract;

The inhibitory effect of AMP-activated protein kinase (AMPK) on chemokine and prostaglandin production in human endometrial stromal cells: Y. Kawano, et al.; Reprod. Biol. Endocrinol. 19, 188 (2021),


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