Porcine pancreatic elastase has a molecular weight of 25.9 kDa, and a pH optimum of 8.5. ?While elastase will hydrolyze a wide variety of protein substrates, it is unique among proteases in its ability to hydrolyze native elastin, a substrate not attacked by trypsin, chymotrypsin or pepsin. Soybean trypsin inhibitor and kallikrein inhibitor suppress proteolytic but not elastolytic activity. ?Elastase is assayed using a method adapted from that of Feinstein et al., Biochem. Biophys. Res. Comm., 50, 1020 (1973) and using the more soluble substrate of Bieth et al., Biochem. Med., 11, 350 (1974).

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Stability/Storage: Elastase is unstable at pH?≤?3.5. When stored as a dry powder the enzyme is stable for 6-12 months at 2-8°C. ?Elastase product codes: ES and ESL have poor solubility at neutral pH and at concentrations greater than 0.25%. ?It is helpful to make primary solutions in KCl or alkaline buffers before diluting into the reaction mixtures or media, compensating for ionic strength or pH changes. ?Stable at pH 4.0-10.4.

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Technical Notes:?1 SucAla3NA unit is approximately equivalent to 6 elastin digestion units. Aqueous liquid suspensions should be aseptically handled to avoid bacterial contamination. Due to the viscous nature of the aqueous suspension (Code: ES) the vial should be rinsed to recover contents.

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Unit Definition: One Unit cleaves one micromole of N-succinyl-L-alanyl-L-alanyl-L-alanine-p-nitroanilide per minute at 25°C, pH 8.0.

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艾美捷Worthington彈性蛋白酶的應(yīng)用：

組織解離：因為彈性蛋白在結(jié)締組織的彈性纖維中的濃度最高，所以經(jīng)常使用彈性蛋白酶解離含有廣泛細胞間纖維網(wǎng)絡(luò)的組織。為此，它通常與其他酶一起使用，例如膠原酶、胰蛋白酶和胰凝乳蛋白酶。

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彈性蛋白酶相關(guān)研究：

膠原酶?(CLS1-4/CLSPA)

脫氧核糖核酸酶?I (DP/D/DCLS/D2/DPFF/DPRF)

透明質(zhì)酸酶?(HSE/HSEP)

中性蛋白酶（分散酶，NPRO）

木瓜蛋白酶?(PAP/PAPL/PAP2)

胃蛋白酶?(PM)

蛋白酶?K (PROK)

胰蛋白酶?(TL/TRL/TRL3/TRLS/TRTPCK)

胰蛋白酶抑制劑?(LBI/OI/SI/SIC)

細胞分離優(yōu)化系統(tǒng)?(CIT)

肝細胞分離系統(tǒng)?(HIS)

新生兒心肌細胞分離系統(tǒng)?(NCIS)

木瓜蛋白酶解離系統(tǒng)?(PDS/PDS2)